Pitfalls in using synthetic substrates for the diagnosis of Gaucher's disease. ] an enzyme of the hydrolase class that splits glucoside, occurring as α-, β-, and α-1,3-glucosidase; α-glucosidase occurs in intestinal juice, and β-glucosidase in the kidney, liver, and intestinal mucosa. Two proposed mechanisms include a nucleophilic displacement and an oxocarbenium ion intermediate. Alpha-glucosidase breaks down starch and disaccharides to glucose. Maltase, an analogous enzyme that cleaves maltose, is nearly functionally equivalent. reesei strains might be developed producing distinctive combination of cellulases which could possibly be utilized for different application in trade. Properties of a novel glucose-enhanced beta-glucosidase purified from Streptomyces sp. Purification and characterization of a particularly thermostable beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus.
bAmount of enzyme releasing 1 μmol product from first-listed substrate per minute. Substrate chosen corresponds to that giving largest reported specific activity. https://enzymes.bio/ , characterization, gene cloning, and sequencing of a new beta-glucosidase from Bacillus circulans subsp. Extraction, partial purification and willpower of some biochemical properties of β-glucosidase from Tea Leaves (Camellia sinensis L.). Stability and substrate specificity of a beta-glucosidase from the thermophilic bacterium Tp8 cloned into Escherichia coli. Comparison of assorted beta-glucosidase assays used to diagnose Gaucher's illness. Korotkova OG, Semenova MV, Morozova VV, Zorov IN, Sokolova LM, Bubnova TM, Okunev ON, Sinitsyn AP. Isolation and properties of fungal β-glucosidases. Karnaouri A, Topakas E, Paschos T, Taouki I, Christakopoulos P. Cloning, expression and characterization of an ethanol tolerant GH3 β-glucosidase from Myceliophthora thermophila. Chauve M, Mathis H, Huc D, Casanave D, Monot F, Ferreira NL. Comparative kinetic evaluation of two fungal β-glucosidases. Öhgren K, Vehmaanperä J, Siika-aho M, Galbe M, Viikari L, Zacchi G. High temperature enzymatic prehydrolysis prior to simultaneous saccharification and fermentation of steam pretreated corn stover for ethanol manufacturing. Screening of β-glucosidase enzymes for elevated thermostability can be performed in a manner very similar to that described in the above sections for the endoglucanase. thermocellum was thus elevated utilizing both random mutagenesis and alignment-guided mutagenesis. The following protocol was used to screen and select the thermostable variants. Colonies that remain purple after the warmth therapy probably comprise thermostable enzymes. Two management plates containing wild-kind bglA ought to be prepared every time and used with and without the warmth problem. Hong J, Tamaki H, Kumagai H. Cloning and useful expression of thermostable β-glucosidase gene from Thermoascus aurantiacus. Tong CC, Cole AL, Shepherd MG. Purification and properties of the cellulases from the thermophilic fungus Thermoascus aurantiacus. Kengen SWM, Luesink EJ, Stams AJM, Zehnder AJB. Purification and characterization of an especially thermostable β-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus. Christakopoulos P, Goodenough PW, Kekos D, Macris BJ, Claeyssens M, Bhat MK. Purification and characterization of an extracellular β-glucosidase with transglycosylation and exo-glucosidase activities from Fusarium oxysporum. Chirico WJ, Brown RD. Purification and characterization of a β-glucosidase from Trichoderma reesei.
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